Microvolume, kinetic-dependent enzyme-linked immunosorbent assay for amoeba antibodies.


We describe a microvolume enzyme-linked immunosorbent assay based on enzyme rate kinetics. Antigens from Entamoeba histolytica were adsorbed in wells of disposable polystyrene strips containing 12 flat-bottom wells. After exposure to the serum of a patient and peroxidase-labeled anti-human immunoglobulin G, the rate of color change in specific substrate was determined by eight sequential readings of individual wells over a 2-min period with a microcomputer-controlled model MR-600 automated plate reader. The changes in absorbance readings were converted to slope values for each well by the microcomputer. Thus, 12 samples were read, and results were printed in ca. 3.5 min. Assay conditions are described and data are presented to show that this assay is quantitative for antibody and antigen concentration with a single-tube (well) dilution.


Citations per Year

119 Citations

Semantic Scholar estimates that this publication has 119 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Mathews1984MicrovolumeKE, title={Microvolume, kinetic-dependent enzyme-linked immunosorbent assay for amoeba antibodies.}, author={Harley Mathews and Kenneth W Walls and A Y Huong}, journal={Journal of clinical microbiology}, year={1984}, volume={19 2}, pages={221-4} }