Microtubules are stabilized in confluent epithelial cells but not in fibroblasts

  title={Microtubules are stabilized in confluent epithelial cells but not in fibroblasts},
  author={Rainer Pepperkok and M. H. Br{\'e} and J Davoust and Thomas E. Kreis},
  journal={The Journal of Cell Biology},
  pages={3003 - 3012}
Rhodamine-tagged tubulin was microinjected into epithelial cells (MDCK) and fibroblasts (Vero) to characterize the dynamic properties of labeled microtubules in sparse and confluent cells. Fringe pattern fluorescence photobleaching revealed two components with distinct dynamic properties. About one-third of the injected tubulin diffused rapidly in the cytoplasm with a diffusion coefficient of 1.3-1.6 x 10(-8) cm2/s. This pool of soluble cytoplasmic tubulin was increased to greater than 80% when… CONTINUE READING


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Publications referenced by this paper.
Showing 1-10 of 69 references

Fluorescent micrombules break up under illumination

G.P.A. Vigers, M. Cone, J. R. McIntosh.
J. Cell Biol. 107:1011-1024. • 1988
View 4 Excerpts
Highly Influenced

Role of microtubules in the organisation of the Golgi apparatus.

Cell motility and the cytoskeleton • 1990
View 1 Excerpt

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