Microtubule polymerization dynamics.

@article{Desai1997MicrotubulePD,
  title={Microtubule polymerization dynamics.},
  author={Arshad Desai and Timothy J. Mitchison},
  journal={Annual review of cell and developmental biology},
  year={1997},
  volume={13},
  pages={
          83-117
        }
}
The polymerization dynamics of microtubules are central to their biological functions. Polymerization dynamics allow microtubules to adopt spatial arrangements that can change rapidly in response to cellular needs and, in some cases, to perform mechanical work. Microtubules utilize the energy of GTP hydrolysis to fuel a unique polymerization mechanism termed dynamic instability. In this review, we first describe progress toward understanding the mechanism of dynamic instability of pure tubulin… 

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References

SHOWING 1-10 OF 189 REFERENCES

Dynamic instability of microtubules

TLDR
The basic concepts of this model provide a framework for future characterization of the molecular basis of spatial and temporal regulation of micro Tubulin dynamics in the cell and the function of microtubule dynamics in motile processes such as chromosome movement.

Modulation of microtubule dynamics during the cell cycle.

  • F. McNally
  • Biology, Environmental Science
    Current opinion in cell biology
  • 1996

Regulation of microtubule dynamic instability.

TLDR
An understanding of how these proteins function may provide further insight into how cells organize the different arrays of microtubules used for such processes as vesicle transport, polarized organization of organelles, and chromosome movement.

Microtubule dynamics modulated by guanosine triphosphate hydrolysis activity of beta-tubulin.

TLDR
It is demonstrated that the rate of GTP hydrolysis can modulate microtubule length and hence dynamic instability, and this work confirmed the role of yeast beta-tubulin in this instability.

Modulation of the dynamic instability of tubulin assembly by the microtubule-associated protein tau.

TLDR
Tau generates a partially stable but still dynamic state in microtubules, which is perturbed by phosphorylation by MAP2 kinase, which affects all three activities by lowering the affinity of tau for the microtubule lattice.

Microtubule Treadmilling in Vivo

TLDR
In cytoplasmic fragments of fish melanophores, microtubules were shown to detach from their nucleation site and depolymerize from their minus ends and permit a minus-end mechanism of microtubule turnover and treadmilling.

Direct observation of microtubule dynamics in living cells

TLDR
Direct observations of fluorescent microtubules in the fibroblast lamellum show that individualmicrotubules undergo rounds of assembly and disassembly from the same end, which is consistent with dynamic instability and seeks to directly test the mechanism of turnover.

Microtubule dynamics and microtubule caps: a time-resolved cryo- electron microscopy study

Force generation by microtubule assembly/disassembly in mitosis and related movements.

TLDR
The dynamic nature of the filaments (microtubules) that make up the labile fibers of the mitotic spindle and asters are reviewed and how such assembling and disassembly of polymer filaments can generate forces that are utilized by the living cell in mitosis and related movements is considered.
...