Microtubule network is required for insulin signaling through activation of Akt/protein kinase B: evidence that insulin stimulates vesicle docking/fusion but not intracellular mobility.

@article{Eyster2006MicrotubuleNI,
  title={Microtubule network is required for insulin signaling through activation of Akt/protein kinase B: evidence that insulin stimulates vesicle docking/fusion but not intracellular mobility.},
  author={Craig A Eyster and Quwanza S Duggins and Gary J Gorbsky and Ann Louise Olson},
  journal={The Journal of biological chemistry},
  year={2006},
  volume={281 51},
  pages={39719-27}
}
The microtubule network has been shown to be required for insulin-dependent GLUT4 redistribution; however, the precise molecular function has not been elucidated. In this article, we used fluorescence recovery after photobleaching (FRAP) to evaluate the role of microtubules in intracellular GLUT4 vesicle mobility. A comparison of the rate of fluorescence recovery (t((1/2))), and the maximum fluorescence recovered (F(max)) was made between basal and insulin-treated cells with or without… CONTINUE READING