Microtubule-dependent microtubule nucleation based on recruitment of gamma-tubulin in higher plants.

  title={Microtubule-dependent microtubule nucleation based on recruitment of gamma-tubulin in higher plants.},
  author={Takashi Murata and Seiji Sonobe and Tobias I. Baskin and Susumu Hyodo and Seiichiro Hasezawa and Toshiyuki Nagata and Tetsuya Horio and Mitsuyasu Hasebe},
  journal={Nature cell biology},
  volume={7 10},
Despite the absence of a conspicuous microtubule-organizing centre, microtubules in plant cells at interphase are present in the cell cortex as a well oriented array. A recent report suggests that microtubule nucleation sites for the array are capable of associating with and dissociating from the cortex. Here, we show that nucleation requires extant cortical microtubules, onto which cytosolic gamma-tubulin is recruited. In both living cells and the cell-free system, microtubules are nucleated… CONTINUE READING
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