Microtubule cytoskeleton and morphogenesis in the amoebae of the myxomycete Physarum polycephalum

  title={Microtubule cytoskeleton and morphogenesis in the amoebae of the myxomycete Physarum polycephalum},
  author={Michel Wright and Catherine Albertini and V. Planques and Isabelle Meynial Salles and Bernard Ducommun and Catherine Gely and Haleh Akhavan-Niaki and Luis Mir and André Moisand and M. L. Oustrin},
  journal={Biology of the Cell},

Microtubule organization during the cell cycle of the primitive eukaryote dinoflagellate Crypthecodinium cohnii.

The complete microtubular system of the dinoflagellate Crypthecodinium cohnii Biecheler is described, as seen by confocal laser scanning fluorescence microscopy and labelling with anti-beta-tubulin

Building a plasmodium: Development in the acellular slime mould Physarum polycephalum

With the introduction of a DNA transformation system for P. polycephalum, it is now possible to investigate the functions of genes in the vegetative cell types and their roles in the cellular reorganisations accompanying development.


The flagellar apparatus of the marine dinoflagellate Amphidinium rhynchocephalum Anissimowa was examined using the techniques of rapid freezing/freeze substitution and serial thin section three dimensional reconstruction to discuss the possible function and evolutionary importance of these structures.

An ultrastructural and molecular study of Hyperamoeba dachnaya, n. sp., and its relationship to the mycetozoan slime moulds

The study reveals extensive similarities between Hyperamoeba and the flagellated stages of myxogastrids, including mitochondria with tubular cristae and an electron-dense central body.


The combined presence of wellveloped striated collars, a striated collar connective, and a basal body angle of approximately 120° indicates that this flagellar apparatus is most like that described for Peridinioid dinoflagellates.

Polypeptides from the myxomycete Physarum polycephalum interacting in vitro with microtubules.

The presence in Physarum amoebal crude extracts of at least six polypeptides that bind specifically to amoEBal microtubules and the binding between these proteins and mammalian microtubule-interacting proteins is shown for the first time.



Spatial relationships between the anterior centriole and the mitotic center during interphase in the Amoebae of the Myxomycete Physarum polycephalum

Each pair of centrioles constitutes a morphological and physiological entity that is distinct from the mitotic center (mtoc 1) and the attachment of the anterior centriole to the mtoc 1 occurs at the end of each mitosis.

Identification of a Microfilament-Enriched, Motile Domain in Amoeboflagellates of Physarum Polycephalum

The ridge constitutes a new system for studying the structural basis of cell motility, and the arrangements of actin- and microtubule-containing ridge components suggest several testable hypotheses concerning their involvement in cytoskeletal or motile functions.

Microtubule nucleation by the isolated microtubule-organizing centre of Physarum polycephalum myxamoebae.

The nucleus--centrosome complex from Physarum polycephalum myxamoebae has been purified and endogenous microtubule arrays had been stripped from the complex during isolation.

Identification and characterization of microtubule proteins from myxamoebae of Physarum polycephalum.

The mitotic inhibitor griseofulvin (7-chloro-2',4,6-trimethoxy-6'-methylspiro[benzofuran-2(3H),1'-cyclohex-2'-ene] -3,4'-dione) affected co-polymer microtubule protein in a similar way, but to a slightly greater extent.

Distribution of acetylated alpha-tubulin in Physarum polycephalum

A monoclonal antibody specific for acetylated alpha-tubulin, 6-11B-1, was used to screen for this protein during three different stages of the Physarum life cycle-- the amoeba, the flagellate, and the plasmodium.

The development of flagella in swarm cells of the myxomycete Physarum flavicomum.

  • H. Aldrich
  • Biology
    Journal of general microbiology
  • 1968
Myxamoebae of Physarum flavicomum and the stages of their development of flagella during transformation into biflagellate swarm cells were examined with the electron microscope, finding the flagellation process was similar to that reported in other systems.

A comparative study of mitosis in amoebae and plasmodia of the true slime mold Didymium nigripes.

  • S. Kerr
  • Biology
    The Journal of protozoology
  • 1967
SYNOPSIS. Studies comparing mitosis in amoebae and plasmodia of the true slime mold Didymium nigripes reveal that at the time of differentiation pronounced changes occur in the mitotic process. Not

A gene encoding the major beta tubulin of the mitotic spindle in Physarum polycephalum plasmodia.

It is suggested that beta-tubulins diverge more when their expression pattern is restricted, especially when this restriction results in their use in fewer functions, and this divergence among beta tubulins could have resulted through neutral drift.