Microtubule Interactions with the Cell Cortex Causing Nuclear Movements in Saccharomyces cerevisiae

@article{Adames2000MicrotubuleIW,
  title={Microtubule Interactions with the Cell Cortex Causing Nuclear Movements in Saccharomyces cerevisiae},
  author={Neil R. Adames and John A Cooper},
  journal={The Journal of Cell Biology},
  year={2000},
  volume={149},
  pages={863 - 874}
}
During mitosis in budding yeast the nucleus first moves to the mother-bud neck and then into the neck. Both movements depend on interactions of cytoplasmic microtubules with the cortex. We investigated the mechanism of these movements in living cells using video analysis of GFP-labeled microtubules in wild-type cells and in EB1 and Arp1 mutants, which are defective in the first and second steps, respectively. We found that nuclear movement to the neck is largely mediated by the capture of… CONTINUE READING

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