Microtubule Formation in vitro in Solutions Containing Low Calcium Concentrations

@article{Weisenberg1972MicrotubuleFI,
  title={Microtubule Formation in vitro in Solutions Containing Low Calcium Concentrations},
  author={Richard C. Weisenberg},
  journal={Science},
  year={1972},
  volume={177},
  pages={1104 - 1105}
}
Isolated rat brain tubulin can be repolymerized in vitro in solutions containing adenosine triphosphate or guanosine triphosphate, magnesium ions, and a good calcium chelator. The extreme sensitivity of tubulin to calcium ions explains the failure of previous efforts to obtain polymerization and suggests a possible mechanism for regulation of microtubule polymerization in vivo. 

Microtubule formation by pure brain tubulin in vitro. The influence of dextran and poly(ethylene glycol).

Homogeneous brain tubulin, free of microtubule associated proteins, forms microtubules in polymerization buffers containing 7.5--15% dextran T10 or 2--4.5% poly(ethylene glycol) type 6000, which are sensitive to colchicine and low temperature.

Alterations in number of protofilaments in microtubules assembled in vitro

Tubulin from bovine brain was polymerized in vitro using a variety of assembly conditions. Many of the formed microtubules were shown to contain 14 wall protofilaments. The number of microtubules

Pressure-induced depolymerization of brain microtubules in vitro.

Brain microtubules assembled in vitro from tubulin extracted from rabbit brain were subjected to changes in hydrostatic pressure and temperature, and the characteristic response of brain micro Tubulin in vitro to pressure is similar to that of mitotic spindle microtubule in vivo.

Interaction of Calcium-Calmodulin in Microtubule Assembly in Vitro

In most eukaryotic cells, microtubules, intermediate filaments, and microfilaments constitute a fibrous network that is termed the cytoskeleton. Microtubules have been implicated in a number of cell
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