Microtiter plate monoclonal antibody epitope analysis of Ca2+- and Mg2+-induced conformational changes in troponin C.

@article{Jin2007MicrotiterPM,
  title={Microtiter plate monoclonal antibody epitope analysis of Ca2+- and Mg2+-induced conformational changes in troponin C.},
  author={Jian-Ping Jin and Stephen M. Chong and Muhammad M. Hossain},
  journal={Archives of biochemistry and biophysics},
  year={2007},
  volume={466 1},
  pages={1-7}
}
Spectroscopic methods such as circular dichroism and Förster resonance energy transfer are current approaches for monitoring protein conformational changes. Those analyses require special equipment and expertise. The need for fluorescence labeling of the protein may interfere with the native structure. We have developed a microtiter plate-based monoclonal antibody (mAb) epitope analysis to detect protein conformational changes in a high throughput manner. This method is based on the concept… CONTINUE READING