Microtiter plate assay for the measurement of glutathione and glutathione disulfide in large numbers of biological samples.

@article{Baker1990MicrotiterPA,
  title={Microtiter plate assay for the measurement of glutathione and glutathione disulfide in large numbers of biological samples.},
  author={Margaret A. Baker and George J. Cerniglia and Aysha Zaman},
  journal={Analytical biochemistry},
  year={1990},
  volume={190 2},
  pages={360-5}
}
By combining the least complicated and expedient methods of sample handling with the sensitivity and specificity of the GSH assay by enzymatic recycling and the small volumes and software capabilities of microtiter plate technology we have devised a rapid, sensitive, and easy assay for GSH and GSSG in biological samples. The assay is sensitive to 5 pmol in sample volumes of 50 microliters, although other volumes could be used. The use of a computer-driven microplate with software capable of… CONTINUE READING
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