Microsurgically fused human somatic cell hybrids: analysis and cloning.

  • E. G. Diacumakos
  • Published 1973 in
    Proceedings of the National Academy of Sciences…


Cell fusion by microsurgery permits production of specified bicellular hybrid cells with very high efficiency. Systematic analysis of the fate of bicellular hybrids reveals fundamental properties of cells in accordance with cell type. Bicellular hybrids that do not proliferate survive 1-21 days; those that do proliferate form clones 8-9 days after fusion.