Microsomal flavonoid 3'-monooxygenase from maize seedlings.

@article{Larson1986MicrosomalF3,
  title={Microsomal flavonoid 3'-monooxygenase from maize seedlings.},
  author={Russell L. Larson and J. B. Bussard},
  journal={Plant physiology},
  year={1986},
  volume={80 2},
  pages={
          483-6
        }
}
Identification of flavonoid 3'-monooxygenase establishes another reaction in the biosynthesis of flavonoid compounds in maize (Zea mays L.). The flavonoid 3'-hydroxylase was obtained as a microsomal enzyme preparation by buffer extraction of 5 day old maize seedlings and ultracentrifugation. Seedlings were exposed to light 24 hours prior to enzyme extraction. The extraction buffer required the addition of sucrose or glycerin and dithiothreitol to obtain an active hydroxylase that retained its… 

Figures and Tables from this paper

Maize Microsomal Benzoxazinone N-Monooxygenase' 12

Data presented supports the hypothesis that the enzyme has been identified in maize microsomal preparations which catalyzes the N-hydroxylation of HBOA to form DIBOA is a cytochrome P-450 dependent Nmonooxygenase.

Maize microsomal benzoxazinone N-monooxygenase.

Data presented supports the hypothesis that the enzyme has been identified in maize microsomal preparations which catalyzes the N-hydroxylation of HBOA to form DIBOA is a cytochrome P-450 dependent N-monooxygenase.

Characterization of Flavonoid 3 ’ , 5 ’-Hydroxylase in Microsomal Membrane Fraction of Petunia hybrida Flowers ’

Activity varied with the development of flowers, peaking immediately prior to and during anthesis, but was absent in mature flowers, consistent with the suggestion that the P. hybrida F3’,5’H activity was sensitive to low concentrations of all detergents, and therefore solubilization of the active enzyme was not achieved.

Flavonoid hydroxylase from Catharanthus roseus: cDNA, heterologous expression, enzyme properties and cell-type specific expression in plants.

The cell-type specific expression of the P450 dependent flavonoid hydroxylase from the ornamental plant Catharanthus roseus suggests that intercellular transport may play an important role in the compartmentation of the pathways to the different flavonoids.

Proposed biosynthetic pathway for rosmarinic acid in cell cultures of Coleus blumei Benth

A biosynthetic pathway for rosmarinic acid is proposed, deduced from studies of the enzymes detectable in preparations from suspension cells of Coleus blumei, which introduces the hydroxyl groups at positions 3 and 3′ of the aromatic rings of the ester 4-coumaroyl-4′-hydroxyphenyllactate giving rise to ros marinic acid.
...

References

SHOWING 1-10 OF 18 REFERENCES

Induction and characterization of a microsomal flavonoid 3'-hydroxylase from parsley cell cultures.

The results prove that flavonoid 3'-hydroxylase and cinnamate 4-hydroxyase are two different microsomal monooxygenases.

The 4-hydroxylation of cinnamic acid by sorghum microsomes and the requirement for cytochrome P-450.

Genetic Control of Flavanone 3-Hydroxylase Activity and Flavonoid 3′-Hydroxylase Activity in Antirrhinum majus (Snapdragon)

A strict correlation was found between 3-hydroxylase activity and the gene eos which is known to control the hydroxylation of flavones, flavonols and anthocyanins in the 3′-position, similar to those previously obtained with Matthioia incana.

Properties of a Mixed Function Oxygenase Catalyzing Ipomeamarone 15-Hydroxylation in Microsomes from Cut-Injured and Ceratocystis fimbriata-Infected Sweet Potato Root Tissues.

The results suggest that ipomeamarone 15-hydroxylase is a cytochrome P-450-dependent, mixed-function oxygenase.

Anthocyanin Biosynthesis in Flowers of Matthiola incana Flavanone 3-and Flavonoid 3′-Hydroxylases

Abstract Enzyme preparations from flowers of defined genotypes of Matthiola incana contain two dif ferent hydroxylases for hydroxylation of naringenin in the 3-and 3′-position, respectively. The

THE CARBON MONOXIDE-BINDING PIGMENT OF LIVER MICROSOMES. I. EVIDENCE FOR ITS HEMOPROTEIN NATURE.

The present paper gives a detailed account of the investigations on rabbit liver microsomes and crude microsomal digests, which have led to postulate the hemoprotein nature of the pigment.