Microsomal flavonoid 3'-monooxygenase from maize seedlings.

  title={Microsomal flavonoid 3'-monooxygenase from maize seedlings.},
  author={Russell L. Larson and J. B. Bussard},
  journal={Plant physiology},
  volume={80 2},
Identification of flavonoid 3'-monooxygenase establishes another reaction in the biosynthesis of flavonoid compounds in maize (Zea mays L.). The flavonoid 3'-hydroxylase was obtained as a microsomal enzyme preparation by buffer extraction of 5 day old maize seedlings and ultracentrifugation. Seedlings were exposed to light 24 hours prior to enzyme extraction. The extraction buffer required the addition of sucrose or glycerin and dithiothreitol to obtain an active hydroxylase that retained its… 

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