Microsomal conjugation and oxidation of bilirubin.

  title={Microsomal conjugation and oxidation of bilirubin.},
  author={H. T. M. Cuypers and Ernst ter Haar and Petra Lynen Jansen},
  journal={Biochimica et biophysica acta},
  volume={758 2},
Bilirubin diglucuronide and bilirubin monoglucuronide are formed on incubation of microsomal preparations from rat liver with bilirubin and UDPglucuronate. Microsomal diglucuronide formation is a two-step reaction: first monoglucuronide is formed and this is subsequently converted to diglucuronide. Both steps require UDPglucuronate and have a similar pH optimum at pH 7.8. Albumin inhibits the conversion of monoto diglucuronide. Factors favouring diglucuronide formation are: (a) low bilirubin… CONTINUE READING