Microsoft Word - 43940816-file00

Abstract

The environmental toxin and carcinogen 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, dioxin) binds and activates the transcription factor aryl hydrocarbon receptor (AHR) inducing CYP1 family cytochrome P450 enzymes. CYP1A2 and its avian ortholog CYP1A5 are highly active arachidonic acid epoxygenases. Epoxygenases metabolize arachidonic acid to four regioisomeric epoxyeicosatrienoic acids (EETs) and selected monohydroxyeicosatetraenoic acids (HETEs). EETs can be further metabolized by epoxide hydrolases to dihydroxyeicosatrienoic acids (DHETs). As P450-arachidonic acid metabolites affect vasoregulation, responses to ischemia, inflammation and metabolic disorders, identification of their production in vivo is needed to understand their contribution to biologic effects of TCDD and other AHR activators. Here we report use of an acetonitrile-based extraction procedure which markedly increased the yield of arachidonic acid products by lipidomic analysis over a standard solid-phase extraction protocol. and 18-HETE in heart, in a chick embryo model. As the chick embryo heart lacks arachidonic acid metabolizing activity, the latter findings suggest that arachidonic acid metabolites may travel from their site of production to a distal organ, i.e. heart. To determine if the TCDD-arachidonic acid metabolite profile could be altered pharmacologically, chick embryos were treated with TCDD and the soluble epoxide hydrolase inhibitor, AUDA (12-(3-adamantan-1-yl-ureido)-dodecanoic acid). Cotreatment with AUDA increased hepatic EET to DHET ratios, indicating that the in vivo profile of P450-arachidonic acid metabolites can be modified for potential therapeutic intervention. This article has not been copyedited and formatted. The final version may differ from this version.

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Cite this paper

@inproceedings{DianiMoore2013MicrosoftW, title={Microsoft Word - 43940816-file00}, author={Silvia Diani-Moore and Yuliang Ma and Steven S. Gross and Arleen B. Rifkind}, year={2013} }