Microsequencing of proteins recorded in human two-dimensional gel protein databases.

@article{Rasmussen1991MicrosequencingOP,
  title={Microsequencing of proteins recorded in human two-dimensional gel protein databases.},
  author={Helge H. Rasmussen and Jozef Van Damme and Magda Puype and Borb{\`a}la Petrics Gesser and J. E. Celis and Jo{\"e}l Vandekerckhove},
  journal={Electrophoresis},
  year={1991},
  volume={12 11},
  pages={873-82}
}
Sixty-six human proteins recorded in the master transformed human epithelial amnion cells (AMA) (55) and keratinocyte (11) two-dimensional gel protein databases have been microsequenced since the last publication of the AMA database (Electrophoresis 1990, 12, 989-1071). Coomassie Brilliant Blue stained protein spots cut from several (up to 40) dry gels were concentrated by elution-concentration gel electrophoresis, electroblotted onto polyvinylidene difluoride membranes and in situ digested… CONTINUE READING