Microsequences of 145 proteins recorded in the two‐dimensional gel protein database of normal human epidermal keratinocytes

@article{Rasmussen1992MicrosequencesO1,
  title={Microsequences of 145 proteins recorded in the two‐dimensional gel protein database of normal human epidermal keratinocytes},
  author={H. H. Rasmussen and J. Van Damme and M. Puype and B. Gesser and J. Celis and J. Vandekerckhove},
  journal={ELECTROPHORESIS},
  year={1992},
  volume={13}
}
Microsequencing of proteins recovered from two‐dimensional (2‐D) gels is being used systematically to identify proteins in the master human keratinocyte 2‐D gel database. To date, about 250 protein spots recorded in human 2‐D gel databases have been microsequenced and, of these, 145 are recorded in the keratinocyte database under the entry partial amino acid sequence. Coomassie Brilliant Blue‐stained protein spots cut from several (up to 40) dry gels were concentrated by elution‐concentration… Expand
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