Microsequence analysis of the N-terminally blocked proteins immobilized on polyvinylidene difluoride membrane by western blotting.

@article{Hirano1993MicrosequenceAO,
  title={Microsequence analysis of the N-terminally blocked proteins immobilized on polyvinylidene difluoride membrane by western blotting.},
  author={Hironobu Hirano and Setsuko Komatsu and Hideyuki Kajiwara and Yusuke Takagi and Susumu Tsunasawa},
  journal={Electrophoresis},
  year={1993},
  volume={14 9},
  pages={839-46}
}
A technique has been developed for efficient deblocking and subsequent microsequencing of N-terminally blocked proteins immobilized on a polyvinylidene difluoride (PVDF) membrane at the picomole levels. In this technique, proteins were first separated by polyacrylamide gel electrophoresis and then transferred onto a PVDF membrane by Western blotting. The electroblotted proteins with N-terminal acetylserine or acetylthreonine could be deblocked on-membrane by treatment with trifluoroacetic acid… CONTINUE READING