Microscopy of single F(o) F(1) -ATP synthases--the unraveling of motors, gears, and controls.

  title={Microscopy of single F(o) F(1) -ATP synthases--the unraveling of motors, gears, and controls.},
  author={Michael B{\"o}rsch},
  journal={IUBMB life},
  volume={65 3},
Optical microscopy of single F(1) -ATPase and F(o) F(1) -ATP synthases started 15 years ago. Direct demonstration of ATP-driven subunit rotation by videomicroscopy became the new exciting tool to analyze the conformational changes of this enzyme during catalysis. Stimulated by these experiments, technical improvements for higher time resolution, better angular resolution, and reduced viscous drag were developed rapidly. Optics and single-molecule enzymology were entangled to benefit both… CONTINUE READING
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