Microscopic and physiologic evidence for biofilm‐associated wound colonization in vivo

  title={Microscopic and physiologic evidence for biofilm‐associated wound colonization in vivo},
  author={Stephen C. Davis and Carlos A Ricotti and Alex Cazzaniga and Esperanza C. Welsh and William H. Eaglstein and Patricia M. Mertz},
  journal={Wound Repair and Regeneration},
A biofilm is a collection of microbial cells that are attached to a surface and embedded in a self‐produced extrapolymeric substance. The understanding of the biofilm phenotype is important in the understanding of bacteria in vitro but it has been difficult to translate biofilm science to the clinical setting. More recently, preliminary criteria for defining biofilm associated diseases have been proposed and the purpose of this study was to create a biofilm‐associated wound model based on these… 
Use of Tissue‐Engineered Skin to Study In Vitro Biofilm Development
This model may provide a basis on which future studies may explore therapeutic modalities to prevent and eradicate pathogenic bacterial biofilm in humans using Graftskin, a tissue‐engineered skin equivalent.
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A highly quantitative, reproducible in vivo biofilm model is established and validated, providing evidence that the biofilm phenotype specifically contributes to profound cutaneous wound healing impairment, and highlights the importance of bacterial biofilms in chronic wound pathogenesis.
Detection of bacterial fluorescence from in vivo wound biofilms using a point‐of‐care fluorescence imaging device
This pre-clinical study is the first to demonstrate the fluorescence detection of bacterial biofilm in vivo using a point-of-care wound imaging device and have implications for clinicians targeting biofilm and may facilitate improved visualisation and removal of biofilms.
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Using human skin‐equivalent tissues, investigators demonstrated that a number of different species can grow on the tissue and elicit an inflammatory response from the tissue, and a full understanding of how biofilms impact wound‐healing cells and host tissues will have a profound effect on how chronic wounds are treated.
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