Microscopic and UPLC–UV–MS analyses of authentic and commercial yohimbe (Pausinystalia johimbe) bark samples

  title={Microscopic and UPLC–UV–MS analyses of authentic and commercial yohimbe (Pausinystalia johimbe) bark samples},
  author={Vijayasankar Raman and Bharathi Avula and Ahmed M. Galal and Yan-Hong Wang and Ikhlas A. Khan},
  journal={Journal of Natural Medicines},
Yohimbine is the major alkaloid found in the stem bark of yohimbe, Pausinystalia johimbe (Rubiaceae), an evergreen tree native to Africa. The objectives of the current study were to provide a detailed anatomy of yohimbe bark, as well as to determine the quantity of yohimbine in the raw yohimbe products sold online. Twelve commercial raw materials of yohimbe were analyzed by microscopic and ultra performance liquid chromatography–UV–MS methods. The study revealed that three samples were probably… 

Pharmaceutical quantities of yohimbine found in dietary supplements in the USA.

Of the 49 brands of supplements labelled as containing yohimbe or yohimbine available for sale from seven major retailers in the USA, only 4.1% provided consumers with both accurate information about the quantity of yOHimbine as well as information about yohIMbe's known adverse effects.

UPLC-DAD Assisted Phytochemical Quantitation Reveals a Sex, Ploidy and Ecogeography Specificity in the Expression Levels of Selected Secondary Metabolites in Medicinal Tinospora cordifolia: Implications for Elites’ Identification Program

The paper shows the first observations of ploidy specificity along with subtle sex and ecogeography influence on the expression levels of secondary metabolome in T. cordifolia.

Authentication of morphological and microscopic features of stem and leaf of Panax quinquefolius L. grown in Ontario, Canada

The number of compound leaf, palisade ratio, vein islet numbers, starch grain, calcium oxalate, cluster crystals and secretory canal characteristics were presented and it was suggested that the stem and leaves could be further researched on biological active effects.

Microscopic Authentication of Commercial Herbal Products in the Globalized Market: Potential and Limitations

These microscopically authenticated commercial herbal products confirm that different magnifying instruments can be used to authenticate crude or processed herbal products traded in the global marketplace.

Chemical Authentication of Botanical Ingredients: A Review of Commercial Herbal Products

This work has reviewed the successful use of different chemical methods for the botanical authentication of 2,386 commercial herbal products, sold in 37 countries spread over six continents, and identified the total absence of labeled botanical ingredients.

Utility of alkaloids as chemical and biomarkers for quality, efficacy, and safety assessment of botanical ingredients.


Reduction in SILD bioavailability was found as a result of interaction of SILD with Horny goat weed, Thai ginseng, Arugula, Black seed, Garden cress, Fenugreek, and Pummelo juice in human and/or animal models.

Overview of the major classes of new psychoactive substances, psychoactive effects, analytical determination and conformational analysis of selected illegal drugs

Abstract The misuse of psychoactive substances is attracting a great deal of attention from the general public. An increase use of psychoactive substances is observed among young people who do not



Analysis of yohimbine alkaloid from Pausinystalia yohimbe by non-aqueous capillary electrophoresis and gas chromatography-mass spectrometry.

The GC-MS and NACE methods were successfully validated and applied to the quantitation of yohimbine and the different selectivity displayed by these techniques allowed different separation profiles that can be useful in phytochemical characterization of the extracts.

Gas chromatographic determination of yohimbine in commercial yohimbe products.

Comparisons of chromatograms of extracts of authentic bark with those of commercial products indicated that, although many products contained measurable quantities of the alkaloid yohimbine, they were largely devoid of the other alkaloids previously reported in this species.

High-Performance Thin-Layer Chromatographic Quantification of Yohimbine in the Stem Bark of Pausinystalia yohimbe

  • Amar SinghN. Alvi
  • Environmental Science
    JPC – Journal of Planar Chromatography – Modern TLC
  • 2011
A simple, sensitive, and reliable normal-phase HPTLC method has been established for quantification of yohimbine in the stem bark of P. yohimbe. A methanol extract of powdered stem bark was used for

Qualitative and quantitative determination of yohimbine in authentic yohimbe bark and in commercial aphrodisiacs by HPLC-UV-API/ MS methods.

The development and validation of a rapid qualitative and quantitative method based on an HPLC-UV-MS technique with atmospheric pressure chemical ionisation and electrospray ionisation for the

Stability indicating assay of yohimbine hydrochloride using high performance liquid chromatography.

2 Abstract: A sensitive method was established for the analysis of yohimbine(YOH) using high performance liquid c hromatography (HPLC) with fluorescence detection. The proposed method i s stability

A High-Performance Liquid Chromatography Assay for Yohimbine HCl Analysis

St stability-indicating high-performance liquid chromatographic (HPLC) assay procedures were established and utilized to analyze the concentration of the drug and showed that the degradation products obtained from stressing yohimbine HCl by heat and extremes in pH did not interfere with the yohimo HCl peak, although the internal standard, caffeine, did show some interference.

A liquid chromatographic procedure for the analysis of yohimbine in equine serum and urine.

A standardbred mare was dosed with 40 mg yohimbine intravenously. Serum and urine samples were collected and analyzed for yohimbine using solvent extraction and reversed-phase high-performance liquid

Simultaneous determination of thirteen plant alkaloids in a human specimen by SPE and HPLC.

A new screening method for the simultaneous determination of thirteen plant alkaloids in a human specimen was developed based on solid-phase extraction and reversed-phase liquid chromatography with photodiode array detection, which enables selective identification as well as accurate and sensitive quantification.

Quantitative and qualitative HPLC analysis of thermogenic weight loss products.

An HPLC qualitative and quantitative method of seven analytes in thermogenic weight loss preparations available on the market using a Waters XTerra RP18 column as the stationary phase and a gradient mobile phase of water and acetonitrile for UV detection is described.