Microscope calibration using laser written fluorescence

@article{Corbett2018MicroscopeCU,
  title={Microscope calibration using laser written fluorescence},
  author={Alexander D. Corbett and Michael Shaw and Andrew Yacoot and Andrew Jefferson and Lothar Schermelleh and T. Wilson and Martin J. Booth and Patrick S. Salter},
  journal={Optics Express},
  year={2018},
  volume={26},
  pages={21887 - 21899}
}
There is currently no widely adopted standard for the optical characterization of fluorescence microscopes. We used laser written fluorescence to generate two- and three-dimensional patterns to deliver a quick and quantitative measure of imaging performance. We report on the use of two laser written patterns to measure the lateral resolution, illumination uniformity, lens distortion and color plane alignment using confocal and structured illumination fluorescence microscopes. 
Microscope calibration protocol for single-molecule microscopy.
TLDR
A method for calibrating a microscope at the nanometer scale, in the sense of determining optical aberrations as revealed by point source localization errors on the order of nanometers, is proposed.
Microscope calibration protocol for single-molecule microscopy
Single-molecule microscopy allows for the investigation of the dynamics of individual molecules and the visualization of subcellular structures at high spatial resolution. For singlemolecule imaging
Spinning disk-remote focusing microscopy
TLDR
Application to live biological samples was demonstrated by acquiring image volumes over a 24 µm axial range at 1 volume/s, allowing for the detection of calcium-based neuronal activity in Platynereis dumerilii larvae.
3D test sample for the calibration and quality control of super-resolution and confocal microscopes
TLDR
A single sample is presented to calibrate microscopes, align their laser beams and measure their point spread function (PSF) in 3D and can be used to evaluate refractive index mismatches as a function of depth quantitatively.
A simple, inexpensive and multi‐scale 3‐D fluorescent test sample for optical sectioning microscopies
TLDR
Here, the multiple uses of a surprisingly versatile and simple 3‐D test sample that can complement existing and much more expensive calibration samples are advocated and demonstrated: commercial tissue paper labeled with a fluorescent highlighter pen.
A simple, inexpensive and multi-scale 3-D fluorescent test sample for optical sectioning microscopies
TLDR
This “primitive” and inexpensive three-dimensional (3-D) test sample is a surprisingly versatile and powerful tool for quality assessment, comparison across microscopes as well as routine metrology for optical sectioning techniques, both for research labs and imaging core facilities.
3D test sample for the calibration and quality control of stimulated emission depletion (STED) and confocal microscopes
TLDR
A new colloidal crystal standard sample is established for the alignment and calibration of confocal and stimulated emission depletion microscopy, adjustable to meet the requirements of different NA objectives and microscopy techniques and additionally can be used to evaluate refractive index mismatches as a function of depth quantitatively.
Superresolution Linear Optical Imaging in the Far Field
The resolution of optical imaging devices is ultimately limited by the diffraction of light. To circumvent this limit, modern super-resolution microscopy techniques employ active interaction with the
Multi-plane remote refocusing epifluorescence microscopy to image dynamic Ca2+ events
TLDR
An epifluoresence-based remote refocussing imaging system that can image each layer at up to 20fps using different dyes and excitation light for each layer, without the requirement for optically sectioning microscopy is designed and implemented.
Multi-plane remote refocusing epifluorescence microscopy to image dynamic Ca2+ events
TLDR
An epifluoresence-based remote refocussing imaging system that can image each layer at up to 20fps using different dyes and excitation light for each layer, without the requirement for optically sectioning microscopy is designed and implemented.
...
...

References

SHOWING 1-10 OF 25 REFERENCES
Measuring and interpreting point spread functions to determine confocal microscope resolution and ensure quality control
This protocol outlines a procedure for collecting and analyzing point spread functions (PSFs). It describes how to prepare fluorescent microsphere samples, set up a confocal microscope to properly
eSIP: A Novel Solution-Based Sectioned Image Property Approach for Microscope Calibration
TLDR
A novel type of calibration concept for sectioned image property (SIP) measurements which is based on fluorescent solution and makes the calibration concept available for a broader number of users is proposed.
Aberration correction for confocal imaging in refractive‐index‐mismatched media
A major limitation to the use of confocal microscopes to image thick biological tissue lies in the dramatic reduction in both signal level and resolution when focusing deep into a
Quantifying distortions in two-photon remote focussing microscope images using a volumetric calibration specimen
TLDR
By first approximating and then compensating the distortion in imaging data from whole heart rodent studies, the variance of sarcomere length (SL) measurements was reduced by almost 50%.
Optimized approaches for optical sectioning and resolution enhancement in 2D structured illumination microscopy
TLDR
A linear reconstruction method is presented that maximizes the axial frequency extent of the combined 2D structured illumination passband along with an empirically optimized approximation to this scheme.
3D Patterning at the Nanoscale of Fluorescent Emitters in Glass
Three-dimensional fluorescent nanostructures are photoinduced by a near-infrared high repetition rate femtosecond laser in a silver-containing femto-photoluminescent glass. By adjusting the laser
Three dimensional laser microfabrication in diamond using a dual adaptive optics system.
TLDR
It is shown that aberration compensation is essential for the generation of controlled micron-scale features at depths greater than 200 μm, and the dual adaptive optics approach demonstrates increased fabrication efficiency relative to experiments using a single adaptive element.
4D Super-Resolution Microscopy with Conventional Fluorophores and Single Wavelength Excitation in Optically Thick Cells and Tissues
TLDR
The use of a combination of conventional near-infrared dyes, such as Alexa 647, Alexa 680 and Alexa 750, all excited with a 671 nm diode laser, enables 3D multi-colour super-resolution imaging of complex biological samples and requires only a single laser.
Transmission and photoluminescence images of three-dimensional memory in vitreous silica
We demonstrate separate readouts of three-dimensional memory by (i) transmission imaging using a conventional optical microscope and (ii) photoluminescence (PL) of the bits created by inducing
...
...