Microscope-assisted UV-initiated preparation of well-defined porous polymer monolithic plugs in glass microchips for peptide preconcentration.


Herein, highly defined monolithic beds were prepared in glass microchips by photopolymerization of ethylene glycol methacrylate phosphate (EGMP), acrylamide, and N,N'-methylenebisacrylamide (BAA) using an epifluorescence microscope as UV-irradiation source. Such a fast and easy method allowed precise control of (i) the edge shape, (ii) the location along… (More)
DOI: 10.1007/s00216-016-0161-1


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