Microscale isoelectric focusing studies of mouse and human hypoxanthine-guanine phosphoribosyl transferases

Abstract

A microscale isoelectric focusing technique has been developed and used to study hypoxanthine-guanine phosphoribosyl transferase (HGPRT; E.C. 2.4.2.8, inosinate-guanylate:pyrophosphate phosphoribosyl transferase) activities in mouse and human cell lines. The enzymes of both mouse and human origin are shown to exhibit considerable heterogeneity, but each type has a unique range of isoelectric pH. The enzyme of a mouse × human hybrid cell line, derived by fusion of HGPRT− parental cells, gives a homogeneous peak of activity, unlike the wild-type enzyme of either parent. The possibility is suggested that this enzyme activity is due to intra-allelic complementation.

DOI: 10.1007/BF00487447

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Cite this paper

@article{Roy1973MicroscaleIF, title={Microscale isoelectric focusing studies of mouse and human hypoxanthine-guanine phosphoribosyl transferases}, author={Kenneth L. Roy and F. H. Ruddle}, journal={Biochemical Genetics}, year={1973}, volume={9}, pages={175-185} }