Microsatellite primers for vulnerable and thriving Acacia (Fabaceae) species from Australia’s arid zone1

@article{Forrest2015MicrosatellitePF,
  title={Microsatellite primers for vulnerable and thriving Acacia (Fabaceae) species from Australia’s arid zone1},
  author={Cairo N. Forrest and David G. Roberts and Andrew J. Denham and David J. Ayre},
  journal={Applications in Plant Sciences},
  year={2015},
  volume={3}
}
Premise of the study: Microsatellite markers were developed for the common arid Australian shrub Acacia ligulata (Fabaceae) and the threatened overstory trees A. melvillei and A. pendula. Methods and Results: DNA sequence data generated by 454 sequencing were used to identify microsatellite nucleotide repeat motifs. Including previously developed primer sets, we report on the development of 10 polymorphic microsatellite loci for each species. Six of these were novel for A. melvillei and A… 
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Microsatellite markers for vulnerable Australian aridzone Acacias
TLDR
Primers for eight microsatellite loci for Acacia carneorum and Acacia loderi allow assessment of the genetic diversity and connectedness of populations, the relative contribution of asexual reproduction to genotypic diversity and population structure, and use of paternity analysis to identify sires of seed within populations known to have set seed in past decades.
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TLDR
A new method for multiplex PCR that facilitates fluorescence-based SSR genotyping and the multiplexed preparation of DNA templates for SNP assays is described, and several advantages of multiplex-ready PCR for SSR and SNP genotypesing are demonstrated and discussed.
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msatcommander: detection of microsatellite repeat arrays and automated, locus‐specific primer design
TLDR
msatcommander is a platform‐independent program designed to search for microsatellite arrays, design primers, and tag primers using an automated routine, using single‐sequence or concatenated, fasta‐formatted files.
Applications in Plant Sciences
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC-BY-NC-SA 4.0), which permits unrestricted noncommercial use and redistribution provided that
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