Microsatellite primers for vulnerable and thriving Acacia (Fabaceae) species from Australia’s arid zone1

  title={Microsatellite primers for vulnerable and thriving Acacia (Fabaceae) species from Australia’s arid zone1},
  author={Cairo N. Forrest and David G. Roberts and Andrew J. Denham and David J. Ayre},
  journal={Applications in Plant Sciences},
Premise of the study: Microsatellite markers were developed for the common arid Australian shrub Acacia ligulata (Fabaceae) and the threatened overstory trees A. melvillei and A. pendula. Methods and Results: DNA sequence data generated by 454 sequencing were used to identify microsatellite nucleotide repeat motifs. Including previously developed primer sets, we report on the development of 10 polymorphic microsatellite loci for each species. Six of these were novel for A. melvillei and A… 
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Genetic diversity and differentiation of invasive Acacia longifolia in Portugal
The results indicate that differences in phenology and reproductive success observed during a previous study cannot be accounted for by genetic diversity and differentiation alone and suggest a single original introduction and a subsequent acclimation process of A. longifolia.
Highly diverse and highly successful: invasive Australian acacias have not experienced genetic bottlenecks globally.
Levels of genetic diversity are similar in native and invasive populations, and there is little evidence of invasive acacia populations being extensively inbred.
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Microsatellite markers for vulnerable Australian aridzone Acacias
Primers for eight microsatellite loci for Acacia carneorum and Acacia loderi allow assessment of the genetic diversity and connectedness of populations, the relative contribution of asexual reproduction to genotypic diversity and population structure, and use of paternity analysis to identify sires of seed within populations known to have set seed in past decades.
Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping
A new method for multiplex PCR that facilitates fluorescence-based SSR genotyping and the multiplexed preparation of DNA templates for SNP assays is described, and several advantages of multiplex-ready PCR for SSR and SNP genotypesing are demonstrated and discussed.
GENCLONE : a computer program to analyse genotypic data, test for clonality and describe spatial clonal organization
GENCLONE 1.0 is designed for studying clonality and its spatial components using genotype data with molecular markers from haploid or diploid organisms with adapted spatial autocorrelation methods and clonal subrange estimates.
msatcommander: detection of microsatellite repeat arrays and automated, locus‐specific primer design
msatcommander is a platform‐independent program designed to search for microsatellite arrays, design primers, and tag primers using an automated routine, using single‐sequence or concatenated, fasta‐formatted files.
Applications in Plant Sciences
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC-BY-NC-SA 4.0), which permits unrestricted noncommercial use and redistribution provided that
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