Microsatellite markers for the endangered root holoparasite Dactylanthus taylorii (Balanophoraceae) from 454 pyrosequencing.

@article{McLay2012MicrosatelliteMF,
  title={Microsatellite markers for the endangered root holoparasite Dactylanthus taylorii (Balanophoraceae) from 454 pyrosequencing.},
  author={T. G. B. McLay and J. Tate and V. V. Symonds},
  journal={American journal of botany},
  year={2012},
  volume={99 8},
  pages={
          e323-5
        }
}
  • T. G. B. McLay, J. Tate, V. V. Symonds
  • Published 2012
  • Biology, Medicine
  • American journal of botany
  • PREMISE OF THE STUDY Microsatellite loci were isolated and developed as polymorphic markers for the New Zealand endemic root holoparasite Dactylanthus taylorii for use in population and conservation genetics studies. METHODS AND RESULTS Shotgun 454 pyrosequencing was performed on genomic DNA pooled from three individuals of D. taylorii. From 61709 individual sequence reads, primers for 753 microsatellite loci were developed in silico and 72 of these were tested for consistent amplification… CONTINUE READING
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