Microsatellite markers for the Mallorcan midwife toad Alytes muletensis

@article{KraaijeveldSmit2003MicrosatelliteMF,
  title={Microsatellite markers for the Mallorcan midwife toad Alytes muletensis},
  author={Femmie J. L. Kraaijeveld-Smit and Graham Hamilton Rowe and Trevor J. C. Beebee and Richard A. Griffiths},
  journal={Molecular Ecology Notes},
  year={2003},
  volume={3},
  pages={152-154}
}
The highly endangered midwife toad Alytes muletensis occurs only on the Mediterranean island of Mallorca. Little is known about genetic variability and dispersal among the 19 breeding sites (six of which stem from reintroductions) of this amphibian. The value of such information should be high for successful management of this species. We report here the characterization of eight polymorphic microsatellite loci in A. muletensis using individuals from a single population. 

Tables from this paper

Low gene flow but high genetic diversity in the threatened Mallorcan midwife toad Alytes muletensis
TLDR
Fine‐scale genetic structuring in the rare and vulnerable Mallorcan midwife toad Alytes muletensis is investigated using eight polymorphic microsatellite markers and tadpoles from 14 pools covering 10 natural sites and two reintroduction sites were sampled.
Population structure and its implications for conservation of the great silver beetle Hydrophilus piceus in Britain
1. The great silver water beetle Hydrophilus piceus is one of the largest aquatic insects in Europe. In Britain it is rare and endangered, and confined to a small number of low-lying marshes. Very
Isolation and characterization of two dinucleotide and four tetranucleotide polymorphic microsatellite loci in the Iberian midwife toad Alytes cisternasii
TLDR
Development of primers for the amplification by the polymerase chain reaction (PCR) of six polymorphic microsatellite loci in A. cisternasii is described.
Small Scale Population Structure in the Treefrog Bokermannohyla saxicola (Bokermann, 1964) (Anura, Hylidae)
TLDR
Microsatellite markers revealed lack of pairwise genetic differentiation in spatially close demes and also in some distant ones, a pattern that may be caused by the combination of limited migration ability and past event effects contingent upon suitable habitat conditions for frog movement.
High levels of population subdivision in a morphologically conserved Mediterranean toad (Alytes cisternasii) result from recent, multiple refugia: evidence from mtDNA, microsatellites and nuclear genealogies
TLDR
The Iberian Midwife toad is a morphologically conserved species endemic to southwestern and central Iberia and a typical inhabitant of Mediterranean habitats, with particular interest in the increasing relevance of Mediterranean refugia for the survival of genetically differentiated populations during the Pleistocene glaciations as revealed by studies in co‐distributed taxa.
Captive breeding and the fitness of reintroduced species: a test of the responses to predators in a threatened amphibian
TLDR
It is unclear how long-term captive breeding can influence fitness attributes such as natural defences to predators, but efforts are made to establish a baseline for this type of research.
Assessing the robustness of networks of spatial genetic variation.
TLDR
This work extends recent work on the analysis of networks of spatial genetic variation to address the robustness of these networks in the face of perturbations and illustrates the potential of this framework using the case study of an amphibian metapopulation.
Biología y conservación del Ferreret, Alytes muletensis
[spa] El conocimiento de la estructura y dinamica de la poblacion de una especie dada se fundamenta principalmente en el conocimiento de la fraccion adulta. Hasta la fecha dicho conocimiento era

References

SHOWING 1-10 OF 13 REFERENCES
Collecting the Mallorcan midwife toad
  • S. Tonge
  • Environmental Science, Biology
  • 1986
The Mallorcan midwife toad was described, from subfossil remains, as recently as 1977, and it was not until 1980 that some living tadpoles were recognized as belonging to this new species. Once
Molecular Evolutionary Genetics
TLDR
Recent developments of statistical methods in molecular phylogenetics are reviewed and it is shown that the mathematical foundations of these methods are not well established, but computer simulations and empirical data indicate that currently used methods produce reasonably good phylogenetic trees when a sufficiently large number of nucleotides or amino acids are used.
Isolation of human simple repeat loci by hybridization selection.
TLDR
The use of this strategy for the efficient isolation of tandem repeats from other sources of genomic DNA, including DNA from flow-sorted chromosomes, and from other species is anticipated.
Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material.
TLDR
The extraction of DNA from semen and very small bloodstains using Chelex 100 is as efficient or more efficient than using proteinase K and phenol-chloroform extraction and DNA extracted from bloodstain seems less prone to contain PCR inhibitors when prepared by this method.
ANALYZING TABLES OF STATISTICAL TESTS
  • W. Rice
  • Biology, Medicine
    Evolution; international journal of organic evolution
  • 1989
Technique non parametrique pour la signification statistique de tables de tests utilisees dans les etudes sur l'evolution notamment
Breeding the Mallorcan midwife toad Alytes muletensis in captivity
Survival of Baleaphryne
  • Sanchiz and Adrover,
  • 1981
Collecting the Mallorcan midwife
  • toad. Oryx,
  • 1986
...
...