Microsatellite marker development and analysis in the eastern oyster (Crassostrea virginica): confirmation of null alleles and non-Mendelian segregation ratios.

@article{Reece2004MicrosatelliteMD,
  title={Microsatellite marker development and analysis in the eastern oyster (Crassostrea virginica): confirmation of null alleles and non-Mendelian segregation ratios.},
  author={Kimberly S. Reece and Weber L Ribeiro and Patrick M Gaffney and Ryan B. Carnegie and Standish K. Allen},
  journal={The Journal of heredity},
  year={2004},
  volume={95 4},
  pages={346-52}
}
Eighteen microsatellite markers were developed for the Crassostrea virginica nuclear genome, including di-, tri-, and tetranucleotide microsatellite repeat regions that included perfect, imperfect, and compound repeat sequences. A reference panel with DNA from the parents and four progeny of 10 full-sib families was used for a preliminary confirmation of polymorphism at these loci and indications of null alleles. Null alleles were discovered at three loci; in two instances, primer redesign… CONTINUE READING
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