Microsatellite enrichment in organisms with large genomes (Allium cepa L.).

@article{Fischer1998MicrosatelliteEI,
  title={Microsatellite enrichment in organisms with large genomes (Allium cepa L.).},
  author={David Fischer and Konrad Bachmann},
  journal={BioTechniques},
  year={1998},
  volume={24 5},
  pages={
          796-800, 802
        }
}
To exploit the polymorphism of repeat numbers in short tandem repeat (STR) sequences (microsatellites) as molecular markers, STRs must be isolated and PCR primers must be developed in flanking sequences. In species with large genomes such as Allium cepa L. (onion and shallot), an efficient selection procedure for genomic fragments containing STRs is a crucial step. Here we describe a nonradioactive method for microsatellite isolation based on affinity capture of single-stranded restriction… CONTINUE READING
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