Microsatellite DNA in peach (Prunus persica L. Batsch) and its use in fingerprinting and testing the genetic origin of cultivars.

@article{Testolin2000MicrosatelliteDI,
  title={Microsatellite DNA in peach (Prunus persica L. Batsch) and its use in fingerprinting and testing the genetic origin of cultivars.},
  author={Raffaele Testolin and T Marrazzo and Guido Cipriani and Raffaella Quarta and Ignazio Verde and Maria Teresa Dettori and Mariaelena Pancaldi and Silviero Sansavini},
  journal={Genome},
  year={2000},
  volume={43 3},
  pages={512-20}
}
We isolated and sequenced 26 microsatellites from two genomic libraries of peach cultivar 'Redhaven', enriched for AC/GT and AG/CT repeats, respectively. For 17 of these microsatellites, it was possible to demonstrate Mendelian inheritance. Microsatellite polymorphism was assayed in 50 peach and nectarine cultivars. Of the 1300 PCRs carried out, all but two produced amplified products of the expected size. All microsatellites were polymorphic, showing 2-8 alleles per locus. Heterozygosity… CONTINUE READING
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