Microrheometry of semiflexible actin networks through enforced single-filament reptation: frictional coupling and heterogeneities in entangled networks.

@article{Dichtl2002MicrorheometryOS,
  title={Microrheometry of semiflexible actin networks through enforced single-filament reptation: frictional coupling and heterogeneities in entangled networks.},
  author={Marius A Dichtl and Erich Sackmann},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2002},
  volume={99 10},
  pages={6533-8}
}
Magnetic tweezers are applied to study the enforced motion of single actin filaments in entangled actin networks to gain insight into friction-mediated entanglement in semiflexible macromolecular networks. Magnetic beads are coupled to one chain end of test filaments, which are pulled by 5 to 20 pN force pulses through entangled solutions of nonlabeled actin, the test filaments thus acting as linear force probes of the network. The transient filament motion is analyzed by microfluorescence, and… CONTINUE READING