Micropropagation of Venus fly trap by shoot culture

  title={Micropropagation of Venus fly trap by shoot culture},
  author={G. W. Jang and Kwang-Soo Kim and Ro-dong Park},
  journal={Plant Cell, Tissue and Organ Culture},
In order to establish and optimize an in vitro micropropagation protocol of Venus fly trap (Dionaea muscipula Ellis), a carnivorous plant, the effects of medium type, MS medium concentration, pH, and cytokinin and auxin types on shoot proliferation and root formation were investigated using 3-month-old shoots. [] Key Result The shoot proliferation was most effective in 2.3 μM kinetin-supplemented 1/3MS medium at pH 5.5. The best conditions for rooting were 1/3MS medium supplemented with 0.5 μM IBA.
Micropropagation of Penthorum chinense through axillary bud
This study reports a protocol for successful micropropagation of Penthorum chinense using nodal explants on Murashige and Skoog (MS) medium supplemented with 6-benzyladenine (BA) or kinetin (Kn). The
In vitro plantlet production of the endangered Pinguicula vulgaris
This study describes the development of a micropropagation protocol for Pinguicula vulgaris using cultures initiated from in vitro produced seedlings, which successfully acclimatized to ex vitro conditions, exhibiting normal development.
In Vitro Regeneration of Cephalotus follicularis
It is demonstrated that shoot proliferation and survival of C. follicularis is most effective in modified MS (MMS) medium containing one-fifth or one-tenth strength macronutrients and full-strength micronut nutrients.
An improved plant regeneration system and ex vitro acclimatization of Ocimum basilicum L.
Findings indicate that the adaptation of micropropagated plants to ex vitro conditions is more extended in time than generally accepted.
Colchicine and duration time on survival rate and micropropagation of Dionaea muscipula Ellis
Young leaf bases and leaf blades of a carnivorous plant Dionaea muscipula were used as explants for determining the callus multiplication and the survival rate was dependent on the concentration of colchicine and incubation time.
Conservation of holm oak (Quercus ilex) by in vitro culture
In vitro culture techniques are used to propagate tree species, as well as to conserve the species in the short and long term. In the present study, in vitro propagation and conservation of holm oak
Micropropagation of Drosophyllum lusitanicum (Dewy pine), an endangered West Mediterranean endemic insectivorous plant
In this work, in vitro clonal propagation of Drosophyllum lusitanicum (Dewy pine) was obtained from seedlings germinated in vitro, exhibiting normal development.
In vitro propagation of Drosera intermedia in a single step
Shooting proliferation was significantly influenced by Murashige and Skoog macronutrient concentration, showing higher multiplication rates for ¼ MS (the lowest concentration), but was not affected by the addition of 0.1 mg dm−3 kinetin.
An efficient in vitro propagation protocol for Pinguicula lusitanica, a rare insectivorous plant
These plantlets can be used to replenish declining populations in the wild and/or for the extraction of bioactive compounds, reducing pressure on wild stocks.
Micropropagation of Pluchea sagittalis (Lam.) Cabrera
Low salt concentration (25% of the normal concentration) in the medium promoted greater growth of plantlets because the plants had a higher number of roots and longer roots in such an environment.


In vitro propagation of Dionaea muscipula Ellis (Venus Fly Trap)
Micropropagation of Cunila galioides, a popular medicinal plant of south Brazil
Axillary buds of field plants of Cunila galioides Benth were used to evaluate the effect of growth regulators and culture media on the in vitro shoot proliferation and growing, and repeated subcultures of shoot tips and single nodes enabled mass multiplication of shoots without any evidence of decline.
Morphogenetic Responses of Seedlings and Adventitious Buds of the Carnivorous Plant Dionaea muscipula in Aseptic Culture
The morphogenetic responses of Venus's flytrap (Dionaea muscipula) seedlings cultured on Murashige-Skoog medium containing napthaleneacetic acid and benzyladenine include the production of
Source, etiolation and orientation of explants affect in vitro regeneration of Venus fly-trap (Dionaea muscipula)
  • W. Teng
  • Biology
    Plant Cell Reports
  • 1999
The most effective condition for plantlet regeneration was found with etiolated petioles incubated with the adaxial side facing the light, and the site of shoot regeneration on a leaf explant was affected by both illumination and orientation of explants.
Studies on the isolation of plumbagin from in vitro and in vivo grown Drosera species
It is concluded that the extraction of plumbagin from Drosera plants is not commercially feasible.
Nutrient requirements of suspension cultures of soybean root cells.
Drosera spp. (Sundew): Micropropagation and the In Vitro Production of Plumbagin
“Ancient botanical treatises and pharmacopoeias attribute various properties to the sundew, or Drosera, whose red droplets of mucilage do not dry out in the sun. Certain extracts of these plants