The purpose of this study was to establish culture medium requirements for micropropagation of Salvia broussonetii Benth., an important medicinal plant. Cultures were initiated from axillary shoots collected from mature plants. Most satisfactory results were achieved using a MS.2 medium supplemented with 1 mM ascorbic acid, 1.44 μM GA3 and 1.11 μM BAP. Axillary nodes were used for in vitro regeneration of multiple shoots and best results were achieved with MS.2 medium plus 1.44 μM GA3, 2.66 μM BAP and 1.14 μM IAA. Shoots rooted without symptoms of chlorosis or necrosis in half-strength MS.2 medium plus 1.44 μM GA3 and 2.28 μM IAA. Introduction Published research on in vitro culture of Salvia species endemic to the Canary Islands, Spain is limited (Mederos-Molina 1991, 2004b; Mederos-Molina et al. 1994, 1997). So far, there are no reports on in vitro culture and plantlet regeneration in Salvia broussonetii Benth., an endemic shrub species from the Canary Islands. This species is a woody medicinal plant of the family Lamiaceae and it is found across Anaga region and locally abundant, Teno region, Tenerife, Canary Island. This medicinal plant shows a good deal of variation in seed-derived population. It also possesses antioxidant and antifungal chemicals (MederosMolina et al. unpublished data). This paper reports for the first time a protocol for in vitro propagation of S. broussonetii Benth. Materials and Methods The micropropagation of Salvia broussonetii Benth. has been carried out by axillary shoot culture from five-month-old juvenile branches collected from 20-year-old mature plants. Juvenile branches were washed with 1% benomyl *P.O. Box 267: Sebastiana Mederos-Molina, Cultures Technology & Phytopathology Lab., E.T.S. Ingeniería Agraria, La Laguna University and Plant Physiology, Fac. Farmacia, ULL, Tenerife, Canary Islands, Spain [*requests for offprints: email@example.com or La Laguna, (San Cristóbal de), 38200, Apdo. Postal 267, Tenerife, Canary Islands, Spain].