Microprojectile bombardment of plant tissues increases transformation frequency by Agrobacterium tumefaciens

@article{Bidney2004MicroprojectileBO,
  title={Microprojectile bombardment of plant tissues increases transformation frequency by Agrobacterium tumefaciens},
  author={D. Bidney and C. Scelonge and J. Martich and M. Burrus and Lynn Sims and G. Huffman},
  journal={Plant Molecular Biology},
  year={2004},
  volume={18},
  pages={301-313}
}
Bombardment of plant tissues with microprojectiles in an effective method of wounding to promote Agrobacterium-mediated transformation. Tobacco cv. Xanthi leaves and sunflower apical meristems were wounded by microprojectile bombardment prior to application of Agrobacterium tumefaciens strains containing genes within the T-DNA encoding GUS or NPTII. Stable kanamycin-resistant tobacco transformants were obtained using an NPTII construct from particle/plasmid, particle-wounded/Agrobacterium… Expand
127 Citations
Stable genetic transformation of Vigna mungo L. Hepper via Agrobacterium tumefaciens
SAAT: sonication-assisted Agrobacterium-mediated transformation
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 39 REFERENCES
Genetic transformation of strawberry by Agrobacterium tumefaciens using a leaf disk regeneration system
Transgenic tobacco plants and their progeny derived by microprojectile bombardment of tobacco leaves
Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants.
Transfer of foreign genes into intact maize cells with high-velocity microprojectiles.
  • T. Klein, M. Fromm, +4 authors J. Sanford
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1988
...
1
2
3
4
...