Microporation is a valuable transfection method for efficient gene delivery into human umbilical cord blood-derived mesenchymal stem cells

@inproceedings{Lim2010MicroporationIA,
  title={Microporation is a valuable transfection method for efficient gene delivery into human umbilical cord blood-derived mesenchymal stem cells},
  author={Jung Yeon Lim and Sun Hwa Park and Chang Hyun Jeong and Ji Hyeon Oh and Seong Muk Kim and Chung Heon Ryu and Soon A. Park and Jae Geun Ahn and Wonil Oh and Shin Soo Jeun and Jong Wook Chang},
  booktitle={BMC biotechnology},
  year={2010}
}
BACKGROUND Mesenchymal stem cells (MSCs) are an attractive source of adult stem cells for therapeutic application in clinical study. Genetic modification of MSCs with beneficial genes makes them more effective for therapeutic use. However, it is difficult to transduce genes into MSCs by common transfection methods, especially nonviral methods. In this study, we applied microporation technology as a novel electroporation technique to introduce enhanced green fluorescent protein (EGFP) and brain… CONTINUE READING