Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae

  title={Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae},
  author={Hugo Pereira and Lu{\'i}sa Barreira and Andr{\'e} Mozes and Cl{\'a}udia Florindo and Cristina Polo and Catarina V Duarte and Lu{\'i}sa Cust{\'o}dio and Jo{\~a}o Varela},
  booktitle={Biotechnology for biofuels},
We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto solid medium upon FACS can save about 3 weeks during the scale-up process as compared with the growth of the same cultures in liquid medium. This approach enabled us to isolate a biodiverse collection… CONTINUE READING


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