Microplate assay of glutathione s-transferase activity for resistance detection in single-mosquito triturates.

@article{Brogdon1990MicroplateAO,
  title={Microplate assay of glutathione s-transferase activity for resistance detection in single-mosquito triturates.},
  author={William G. Brogdon and Ann Marie Barber},
  journal={Comparative biochemistry and physiology. B, Comparative biochemistry},
  year={1990},
  volume={96 2},
  pages={339-42}
}
1. Optimum conditions are described for a simple, rapid microplate assay that measures glutathione s-transferase (GST) activity accurately and precisely in small portions of single mosquito homogenates. 2. Up to 10 assay replicates were possible for individual adults and larvae. Concentration of GST activity in the head/thorax region allows blood-fed mosquitoes with abdomens removed to be used in assays. 3. The method allows the use of GST activity as a biochemical character in comparative… CONTINUE READING

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