Micropatterning tractional forces in living cells.

  title={Micropatterning tractional forces in living cells.},
  author={Ning Wang and Emanuele Ostuni and George M. Whitesides and Donald E. Ingber},
  journal={Cell motility and the cytoskeleton},
  volume={52 2},
Here we describe a method for quantifying traction in cells that are physically constrained within micron-sized adhesive islands of defined shape and size on the surface of flexible polyacrylamide gels that contain fluorescent microbeads (0.2-microm diameter). Smooth muscle cells were plated onto square (50 x 50 microm) or circular (25- or 50-microm diameter) adhesive islands that were created on the surface of the gels by applying a collagen coating through microengineered holes in an… CONTINUE READING