Micronuclei in mouse bone-marrow cells. A simple in vivo model for the evaluation of drug-induced chromosomal aberrations.

@article{Matter1974MicronucleiIM,
  title={Micronuclei in mouse bone-marrow cells. A simple in vivo model for the evaluation of drug-induced chromosomal aberrations.},
  author={B. Matter and J. Grauwiler},
  journal={Mutation research},
  year={1974},
  volume={23 2},
  pages={
          239-49
        }
}
Abstract For studying, in vivo , chromosomal damage in bone-marrow cells of CD mice the following compounds were used: Trenimon®; Endoxanm® (cyclophosphamide); triethylenemelamine (TEM); methyl methanesulfonate (MMS); ethyl methanesulfonate (EMS); mitomycin C; colchicine; N -methyl- N ′-nitro- N -nitrosoguanidine (MNNG) and caffeine. In a first set of experiments the compounds were given twice intraperitoneally with an interval of 24 h. In a second set, effects on bone marrow were studied after… Expand
Micronucleus test and bone-marrow chromosome analysis: a comparison of 2 methods in vivo for evaluating chemically induced chromosomal alterations.
The sensitivities of 2 cytogenetic tests, chromosome analysis and the micronucleus test, were compared by using mice exposed to the substances methyl methanesulfonate (MMS), mitomycin C (MC) andExpand
Comparative in vivo mutagenicity testing by SCE and micronucleus induction in mouse bone marrow
TLDR
The quantitative comparison indicates that SCE are induced at 1/10–1/100 of the concentrations which are required for the detection of micronuclei. Expand
Characterization of an in vitro micronucleus assay with Syrian hamster embryo fibroblasts.
TLDR
Correlations between the formation of micron nuclei and the Ames test, induction of UDS, cell transformation and the in vivo bone marrow micronucleus test are demonstrated. Expand
Radiation-induced micronucleus formation in mouse bone marrow after low dose exposures.
TLDR
It was observed that the frequency of micronucleated polychromatic erythrocytes (MPCE) increased with the increase in exposure dose at all the post-irradiation time periods studied and the dose-response relationship was linear-quadratic for both MPCE and MNCE. Expand
A correlative study on the genetic damage induced by chemical mutagens in bone marrow and spermatogonia of mice. I. CNU-ethanol.
TLDR
Cytogenetic damage induced by a wide range of concentrations of CNU-ethanol in mice was evaluated by determining the frequencies of micro-nuclei in polychromatic erythrocytes of the bone marrow and three reciprocal translocations, namely between an X chromosome and an autosome. Expand
The induction of chromosomal damage in rat hepatocytes and lymphocytes. I. Time-dependent changes of the clastogenic effects of diethylnitrosamine, dimethylnitrosamine and ethyl methanesulfonate.
TLDR
It is suggested that it is the persistent character of the preclastogenic damage that is responsible for the occurrence of micronucleated hepatocytes at later time intervals after treatment with DEN, rather than the stability ofmicronuclei which might eventually have been formed soon after injection. Expand
AET reduces the frequency of micronuclei in bone marrow cells of mice exposed to gamma radiation.
TLDR
A significant dose response to the irradiation for both treatments and a significant reduction in the frequency of micronucleated cells in the mice receiving the radioprotective agent are shown. Expand
The induction of micronuclei as a measure of genotoxicity. A report of the U.S. Environmental Protection Agency Gene-Tox Program.
TLDR
The most important recommendations in this report are: (1) at least 500 PCE should be examined from each of 8 animals to detect an increase of about 4‰ (per thousand) PCE when the background is less than 4 per 1000, and (2) the highest possible doses should be used. Expand
Induction of micronuclei in mouse fetal liver after exposure in utero to N-methyl-N'-nitro-N-nitrosoguanidine.
TLDR
Findings suggest that MNNG might be inactivated in the maternal systemic circulation and that the agent which induces micronuclei might be distributed to the fetuses by diffusion. Expand
Micronuclei in human bone-marrow cells: evaluation of the micronucleus test using human leukemia patients treated with antileukemic agents.
TLDR
Almost the same changes of micronucleus formation that are observed in the mouse micronucus test are produced in human bone marrow by antileukemic drugs--mutagenic compounds--and, if the micron nuclei were scored restrictively in erythroblasts, the application of the micronsucleus test to humanBone marrow would be reasonable. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 20 REFERENCES
Trenimon-induced chromosomal damage in bone-marrow cells of six mammalian species, evaluated by the micronucleus test.
TLDR
The micronucleus test appears to be a useful method offering the advantages of technical simplicity and applicability to a wide range of test mammals, irrespective of a cytologically favorable karyotype. Expand
EARLY AND LATE CYTOLOGIC EFFECTS OF WHOLE BODY IRRADIATION ON HUMAN MARROW.
TLDR
A careful cytologic evaluation of serially aspirated marrow samples during the first hours and days after whole body exposure proves to be an additional important aid in the assessment of the exposed individual and may well prove to be useful in determining the degree of injury and thus the prognosis. Expand
The micronucleus test. Methodological aspects.
TLDR
The simplest scoring procedure, namely relating micronucleated polychromatic erythrocytes to a given total number of ery throatcytes, was very efficient in the range of low mutagenic effects and therefore well suited for safety screening wherease dose-effect studies comprising very high mutagenal effects required the application of a modified method of scoring. Expand
The activity of some nitroso compounds in the mouse dominant-lethal mutation assay. I. Activity of N-nitroso-N-methylurea, N-methyl-N-nitroso-N'-nitroguanidine and N-nitrosomorpholine.
Abstract N -Nitroso- N -methylurea (NMU) at a dose of 50 mg/kg body wt significantly increased the incidence of early foetal death (dominant lethal mutations) when compared with the solvent controlsExpand
Induction of dominant lethal mutations by alkylating agents in male mice.
TLDR
Results are in good agreement with the expected effect of the chemicals based on work with microorganisms and in an equimolar dosage, MMS is about 4 times more effective than EMS in inducing dominant lethal mutations in these cell stages. Expand
Die mutagene Wirkung von Endoxan bei der Maus
TLDR
The statistical comparison of the dead zygotes of the Endoxan experiments with the control group showed, that all stages of the cycle of spermatogenesis were sensitive to the mutagenic action of Endoxans. Expand
Comparison of radiation-and chemically-induced dominant lethal mutations in male mice.
  • U. Ehling
  • Biology, Medicine
  • Mutation research
  • 1971
TLDR
The frequency of radiation-induced dominant lethal mutations in postspermatogonial stages was dose-dependent, and the absence of induced mutations in spermatozoa and late spermatids was typical for the sPermatogenic response after treatment with mytomycin C. Expand
Dominant Lethal Mutations in Mammals
TLDR
The dominant lethal assay in mice, as described in this chapter, meets both speed and simplicity and relevance to problems of human exposure. Expand
Detection of chemical mutagens by the dominant lethal assay in the mouse.
TLDR
A total of 174 test agents, including pharmaceuticals, food additives, pesticides and organic extracts of air and water pollutants, was tested for mutagenicity in mice using the modified dominant lethal assay, with results determined by increased early fetal deaths per pregnancy and, in some instances, also indirectly by reduction in total implants per pregnancy. Expand
The failure of caffeine to induce mutagenic effects or to synergize the effects of known mutagens in mice.
Abstract Caffeine administered to male mice acutely or for 8 wk prior to mating produced no mutagenic effect in the dominant lethal assay, as measured directly by increased early foetal deaths orExpand
...
1
2
...