Micromolar levels of intracellular calcium reduce gap junctional permeability in lens cultures.

@article{Crow1994MicromolarLO,
  title={Micromolar levels of intracellular calcium reduce gap junctional permeability in lens cultures.},
  author={John D. Crow and Michael M. Atkinson and Ross G. Johnson},
  journal={Investigative ophthalmology & visual science},
  year={1994},
  volume={35 8},
  pages={3332-41}
}
PURPOSE To investigate in bovine and embryonic chicken lens cultures the effects of elevated intracellular calcium on the permeability of gap junctions. To determine the changes in intracellular calcium using fura-2. To detect any changes in the phosphorylation of connexin43 after ionophore treatment. METHODS Lucifer yellow was micro-injected into individual cells, and dye spread to neighboring cells was evaluated. Intracellular calcium levels were measured using the calcium indicator, fura-2… CONTINUE READING
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