Micromethods for the characterization of lipid A-core and O-antigen lipopolysaccharide.

  title={Micromethods for the characterization of lipid A-core and O-antigen lipopolysaccharide.},
  author={Cristina L. Marolda and Piya Lahiry and Enrique D. Vin{\'e}s and Soledad Sald{\'i}as and Miguel A Valvano},
  journal={Methods in molecular biology},
Methods for rapid and simple analysis of lipopolysaccharide (LPS) from bacterial whole-cell lysates or membrane preparations have contributed to advancing our knowledge of the genetics of the LPS biogenesis. LPS, a major constituent of the outer membranes in Gram-negative bacteria, has a complex mechanism of synthesis and assembly that requires the coordinated participation of many genes and gene products. This chapter describes a collection of methods routinely used in our laboratory for the… CONTINUE READING

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