Micrometer-scale domains in fibroblast plasma membranes

  title={Micrometer-scale domains in fibroblast plasma membranes},
  author={Elishalom Yechiel and Michael A. Edidin},
  journal={The Journal of Cell Biology},
  pages={755 - 760}
We have used the technique of fluorescence photobleaching recovery to measure the lateral diffusion coefficients and the mobile fractions of a fluorescent lipid probe, 1-acyl-2-(12-[(7-nitro-2-1, 3-benzoxadiazol-4-yl)aminododecanoyl]) phosphatidylcholine (NBD-PC), and of labeled membrane proteins of human fibroblasts. Values for mobile fractions decrease monotonically with increasing size of the laser spot used for the measurements, over a range of 0.35-5.0 microns. Values for NBD-PC diffusion… CONTINUE READING