This paper reports for the first time on net-unidirectional transport of nanobeads by Brownian motion using a periodic 3-phase electrostatic rectification. The transportation of beads is performed in 1 μm deep, 2 or 3 μm wide PDMS microchannels, which constrain three dimensional random motion of nanobeads into 1D fluctuation, so-called tamed Brownian motion. 3 μm wide and 2μm separated ITO electrodes are fabricated by lift-off process on a 24x36 mm coverglass, and a previously fabricated PDMS sheet with microchannels and processed for inlets is aligned and sealed with the electrodes. Diluted fluorescent nanobead, 500 nm in diameter, solution is introduced and equilibrium of the flow is awaited. Electrostatic rectification observations are performed by an inverted microscope. Rectified netunidirectional transport of nanobeads is achieved at 200 mVpp and 400 mVpp at 1 MHz. 2 different beads are transported along the channel over 20 and 10 μm, respectively, in about 100 seconds, while other bead just wandered around in the same microchannel.