Microinjection studies of duck globin messenger RNA translation in human and avian cells.

@article{Stacey1976MicroinjectionSO,
  title={Microinjection studies of duck globin messenger RNA translation in human and avian cells.},
  author={Dennis W. Stacey and Vincent G. Allfrey},
  journal={Cell},
  year={1976},
  volume={9 4 PT 2},
  pages={725-32}
}
Microinjection techniques were used for the study of the translation of heterospecific messenger RNAs in living cells. Duck globin messenger RNAs, in the form of polysomes, messenger ribonucleoprotein particles, or free mRNAs, were injected into cells of human origin (HeLa), and the subsequent appearance and accumulation of avian globin were measured by specific immunofluorescence techniques. Globin accumulated within a high proportion of HeLa cells for at least 25 hr following injection… CONTINUE READING
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