Microinjection of mRNA and morpholino antisense oligonucleotides in zebrafish embryos.

@article{Yuan2009MicroinjectionOM,
  title={Microinjection of mRNA and morpholino antisense oligonucleotides in zebrafish embryos.},
  author={Shiaulou Yuan and Zhaoxia Sun},
  journal={Journal of visualized experiments : JoVE},
  year={2009},
  volume={27}
}
An essential tool for investigating the role of a gene during development is the ability to perform gene knockdown, overexpression, and misexpression studies. In zebrafish (Danio rerio), microinjection of RNA, DNA, proteins, antisense oligonucleotides and other small molecules into the developing embryo provides researchers a quick and robust assay for exploring gene function in vivo. In this video-article, we will demonstrate how to prepare and microinject in vitro synthesized EGFP mRNA and a… CONTINUE READING

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