Microinjection of lentiviral vectors expressing small interfering RNAs directed against laminin receptor precursor mRNA prolongs the pre-clinical phase in scrapie-infected mice.

@article{Pflanz2009MicroinjectionOL,
  title={Microinjection of lentiviral vectors expressing small interfering RNAs directed against laminin receptor precursor mRNA prolongs the pre-clinical phase in scrapie-infected mice.},
  author={Heike Pflanz and Karen Vana and Gerda Mitteregger and Claudia Pace and Diana Messow and Cilli Sedlaczek and Daphne Nikles and Hans A. Kretzschmar and Stefan F T Weiss},
  journal={The Journal of general virology},
  year={2009},
  volume={90 Pt 1},
  pages={269-74}
}
We examined therapeutic in vitro and in vivo approaches using lentivirus-based packaging of small interfering RNAs (siRNAs) targeting the non-integrin laminin receptor mRNA for treatment and prevention of prion disorders. Transfection of N2aSc(+) cells with recombinant plasmids expressing three different siRNAs, significantly reduced both the LRP (laminin receptor precursor) and PrP(Sc) levels by approximately 40-60 %. Stereotactic intracerebral microinjection of recombinant lentiviral vectors… CONTINUE READING