Microinjection and expression of a mouse metallothionein human growth hormone fusion gene in fertilized salmonid eggs

@article{Rokkones1989MicroinjectionAE,
  title={Microinjection and expression of a mouse metallothionein human growth hormone fusion gene in fertilized salmonid eggs},
  author={Erik Rokkones and Peter Alestr{\o}m and H. Skjervold and Kaare M. Gautvik},
  journal={Journal of Comparative Physiology B},
  year={1989},
  volume={158},
  pages={751-758}
}
Using a microinjection method (Rokkones et al. 1985) deoxyribonucleic acid was introduced into fertilized salmonid eggs. The survival rate after a 28 day period was 91% for injected eggs in comparison to non-injected controls. A gene construct containing the mouse metallothionein promoter fused to the human growth hormone structural gene was microinjected either as a supercoiled plasmid or as a linear sequence. In Southern blot analysis of both 5 and 73 day old dissected rainbow trout embryos… CONTINUE READING
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