Microinjected photoreactivating enzymes from Anacystis and Saccharomyces monomerize dimers in chromatin of human cells.

@article{Zwetsloot1985MicroinjectedPE,
  title={Microinjected photoreactivating enzymes from Anacystis and Saccharomyces monomerize dimers in chromatin of human cells.},
  author={J C Zwetsloot and W. Mary Vermeulen and Jan Hendrik Jozef Hoeijmakers and Akira Yasui and Andr{\'e} Eker and Dirk Bootsma},
  journal={Mutation research},
  year={1985},
  volume={146 1},
  pages={
          71-7
        }
}
Photoreactivating enzymes (PRE) from the yeast Saccharomyces cerevisiae and the cyanobacterium Anacystis nidulans have been injected into the cytoplasm of repair-proficient human fibroblasts in culture. After administration of photoreactivation light, PRE-injected cells displayed a significantly lower level of UV-induced unscheduled DNA synthesis (UDS) than non-injected cells. This indicates that monomerization of the UV-induced pyrimidine dimers in the mammalian chromatin had occurred as a… CONTINUE READING
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