Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9

@inproceedings{Nakade2014MicrohomologymediatedEI,
  title={Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9},
  author={Shota Nakade and Takuya Tsubota and Yuto Sakane and Satoshi Kume and Naoaki Sakamoto and Masanobu Obara and Takaaki Daimon and Hideki Sezutsu and Takashi Yamamoto and Tetsushi Sakuma and Ken-Ichi T Suzuki},
  booktitle={Nature communications},
  year={2014}
}
Genome engineering using programmable nucleases enables homologous recombination (HR)-mediated gene knock-in. However, the labour used to construct targeting vectors containing homology arms and difficulties in inducing HR in some cell type and organisms represent technical hurdles for the application of HR-mediated knock-in technology. Here, we introduce an alternative strategy for gene knock-in using transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced… CONTINUE READING
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