Microfluidics for manipulating cells.

@article{Mu2013MicrofluidicsFM,
  title={Microfluidics for manipulating cells.},
  author={Xuan Mu and Wenfu Zheng and Jiashu Sun and Wei Zhang and Xingyu Jiang},
  journal={Small},
  year={2013},
  volume={9 1},
  pages={
          9-21
        }
}
Microfluidics, a toolbox comprising methods for precise manipulation of fluids at small length scales (micrometers to millimeters), has become useful for manipulating cells. Its uses range from dynamic management of cellular interactions to high-throughput screening of cells, and to precise analysis of chemical contents in single cells. Microfluidics demonstrates a completely new perspective and an excellent practical way to manipulate cells for solving various needs in biology and medicine… 

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References

SHOWING 1-10 OF 213 REFERENCES
Drop-based microfluidic devices for encapsulation of single cells.
TLDR
It is shown that single hybridoma cells in 33 pL drops secrete detectable concentrations of antibodies in only 6 h and remain fully viable and highly flexible and adaptable to a variety of cell-based assays.
Microfluidic device for single-cell analysis.
TLDR
A novel microfluidic device constructed from poly(dimethylsiloxane) using multilayer soft lithography technology for the analysis of single cells is developed, achieving significant improvements in reagent consumption, analysis time, and temporal resolution over macroscale alternatives.
Fundamentals of microfluidic cell culture in controlled microenvironments.
TLDR
This tutorial review integrates fundamental principles from cell biology and local microenvironments with cell culture techniques and concepts in microfluidics to synthesize fundamental concepts that would further improve the efficiency of biological research at the microscale.
Droplet Control for Microfluidics
Future applications of microfluidic technology--in which nanoliter quantities of chemicals are processed and reacted, perhaps on an integrated chip--would immensely benefit from exquisite control of
Microfluidics: a new cosset for neurobiology.
TLDR
The intent of this review is to outline recent advances in microfluidic-based applications in neurobiology, with emphasis on neuron culture, neuron manipulation, neural stem cell differentiation, neuropharmacology, neuroelectrophysiology, and neuron biosensors.
A practical guide to microfluidic perfusion culture of adherent mammalian cells.
TLDR
The integrative and systematic treatment of the microfluidic system design and fabrication, cell culture, and micro-assays provides novices with an effective starting point to build and operate a robust microfludic perfusion culture system for various applications.
Cell culture models in microfluidic systems.
TLDR
The characterization of cell culture in microfluidic systems is discussed, important biochemical and physical features of the cell microenvironment are described, and studies of microfluidity cell manipulation in the context of these features are reviewed.
A General Approach for Patterning Multiple Types of Cells Using Holey PDMS Membranes and Microfluidic Channels
A novel method combining microfluidic channels and holey poly(dimethylsiloxane) PDMS membranes is presented for patterning multiple types of cells on a large variety of substrate, including substrate
The origins and the future of microfluidics
The manipulation of fluids in channels with dimensions of tens of micrometres — microfluidics — has emerged as a distinct new field. Microfluidics has the potential to influence subject areas from
...
1
2
3
4
5
...