• Corpus ID: 46741313

Microfluidics for Ultra High-Throughput Experimentation: Droplets, Dots & Photons

  title={Microfluidics for Ultra High-Throughput Experimentation: Droplets, Dots \& Photons},
  author={Andrew J. deMello},
  journal={arXiv: Fluid Dynamics},
  • A. deMello
  • Published 10 January 2018
  • Chemistry, Biology
  • arXiv: Fluid Dynamics
Andrew J. deMello is professor of Biochemical Engineering in the Department of Chemistry and Applied Biosciences at ETH Z\"urich. In this contribution he describes the efforts that his lab has undertaken in developing novel microfluidic systems for molecular and nanomaterial synthesis, droplet-based systems for ultra-high-throughput experimentation and novel optical techniques for sensitive and rapid analysis in small volume environments. 



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A microdroplet dilutor for high-throughput screening.

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Fluorescence-lifetime imaging of DNA-dye interactions within continuous-flow microfluidic systems.

The resolution of emitting states of the DNA-intercalating dye Hoechst 33258 as it binds to large DNA plasmids in the nonequilibrium conditions provided by a simple microfluidic reactor is demonstrated.

Droplet-based microfluidic platform for high-throughput, multi-parameter screening of photosensitizer activity.

It is shown that the droplet-based microfluidic platform can be used to perform concurrent measurements of light and dark toxicity of the PDT agents and that the platform allows simultaneous measurement of experimental parameters that include dark toxicity, photosensitizer concentration, light dose, and oxygenation levels for the development and testing of PDT agents.

High-throughput, quantitative enzyme kinetic analysis in microdroplets using stroboscopic epifluorescence imaging.

This work presents a novel approach for the extensive characterization of enzyme-inhibitor reaction kinetics within a single experiment by tracking individual and rapidly moving droplets as they pass through an extended microfluidic channel.

Fluorescence lifetime imaging of mixing dynamics in continuous-flow microdroplet reactors.

Fluorescence lifetime imaging can be used to reconstruct mixing patterns within a droplet with a time resolution of 5 micros and is demonstrated to be reproducible in form.